Molecular Characterization of Hydrocarbon-utilizing Rhizobacteria from Hydrocarbon-impacted Mangrove Soil in Ogoni Land, Nigeria
Journal of Materials Science Research and Reviews,
Page 21-32
Abstract
Aim: This study investigated the molecular characterization of hydrocarbon-utilizing rhizobacteria isolates from hydrocarbon-impacted mangrove soil from K-Dere Gokana and Gio Tai, Ogoni land, Nigeria.
Study Design: Cross-sectional study.
Place and Duration of Study: K-Dere Gokana and Gio Tai, Ogoni land, Nigeria, between June 2019 and June 2020.
Methodology: Hydrocarbon-utilizing bacteria were isolated from the rhizosphere of hydrocarbon-impacted mangrove soil. Baseline physicochemical and microbiological characteristics of the rhizosphere soil from the contaminated sites were determined. Hydrocarbon utilization was determined turbidometrically using a spectrophotometer and by growth on Bushnell, Haas agar supplemented with 1% crude oil.
Results: Analysis of the rhizosphere soil revealed that the site was contaminated with crude oil with a TPH value of 547.62±40.15 mg/kg. Based on phylogenetic analysis of these 16S rRNA genes the isolates were classified as Bacillus thuringiensis SA2-1; Bacillus velezensis SA11-2; Morganella morganii SA4-3; Serratia ureilytica SB1-4; Serratia marcescens SB5-5 and Serratia nematodiphila SB16-6. The sequences have been deposited under the accession numbers MN631244.1-MN631249.1. These bacteria all demonstrated efficient hydrocarbon utilization in Bushnell medium with 1% crude as the sole carbon source. These bacteria are well documented putative hydrocarbon utilizers.
Conclusion: This study has demonstrated that hydrocarbon utilization from different genera was associated with the rhizosphere of hydrocarbon-impacted mangroves in Gio and K-Dere.
Keywords:
- Mangroves
- hydrocarbon-utilizing bacteria
- rhizobacteria
- molecular characterization.
How to Cite
References
Awari VG, Ibiene AA, Ariole CN, Ezebuiro V. Broad-range pH/temperature-stable cellulase from a novel hydrocarbon contaminated mangrove soil bacterium, Bacilluslicheniformis VVA21. Microbiology Research Journal International. 2018;24 (2):1-14.
Kennedy AC. Rhizosphere and spermosphere In Principles and application of soil microbiology. (Eds). D.M. Sylvia; 1999
Sørensen J. The rhizosphere as a habitat for soil microorganisms. In modern soil microbiology (Eds). van Elsas, J. D., Trevors, J. T. and Wellington, E. M. H. Marcel Dekker, New York. 1997;21–45
Cleyet-Marcel JC, Larcher M, Bertrand H, Rapior S, Pinochet X. Plant growth enhancement by rhizobacteria. In: Nitrogen assimilation by plants. Physiological, biochemical and molecular aspects. Morot-Caudry, J. F. (ed), En field (NH, USA), Plymouth (UK): Science Publishers. 2001;184-197.
Kloepper JW. Plant growth-promoting rhizobacteria. p. In: Okon (ed.), Azospirillum/ Plant Associations, F L, 1994;137-166.
Stewart EA, Grimshaw HM, Parkinson JA, Quarmby C. Chemical Analysis of Ecological Material. Blackwell Scientific, Oxford; 1974
Ho ST, Tsai IJ, Cole KC. Correlation among aqua-regia extractable heavy metals in vertical river sediments. Diffuse Pollution Conference, Dublin. 2003;14:12-18.
APHA. Standard Methods for the Examination of Water and Wastewater (21st ed.). American Public Health Association. Washington, DC; 2005.
Chikere CB, Okpokwasili GC. Lchiakor O. Characterisation of hydrocarbon utilizing bacteria in tropical marine sediments. African Journal of Biotechnology. 2009;8 (11):2541–2544
Ibiene AA, Orji FA, Ezidi CO, Ngwobia CL. Bioremediation of hydrocarbon contaminated soil using spent mushroom compost and other organic wastes. Nigerian. Journal of Food Agriculture and Environment. 2011a;7:1-7.
Abu GO, Chikere BO. Cell surface properties of hydrocarbon – Utilizing bacterial isolates from Port Harcourt marine environment. Nigerian Journal of Microbiology. 2006;20:809-816.
Atuanya EI, Ibeh IN. Bioremediation of crude of oil – Contaminated Loamy, sandy and clay soils Nigerian Journal of Microbiology. 2004;18:373–386.
Chukwura EI, Nwokolo CI, Nwachukwu SCU. Bioremediation of crude oil polluted Escravos River using Candida utilis. Nigerian Journal Microbiology. 2005;19: 623-630.
Chikere BO, Chijioke-Osuji CC. Microbial diversity and physicochemical properties of a crude oil-polluted soil. Nigerian Journal of Microbiology. 2006; 20:1039-1046.
Numbere AO. Impact of hydrocarbon pollution on the mangrove ecosystem of the Niger River Delta, Nigeria. Dissertation, Saint Louis University; 2014.
Okpokwasili GC, Amanchukwu SC. Petroleum hydrocarbon degradation by Candida species. Environment International. 1988;14:243-247.
Mac Faddin JF. Biochemical tests for identification of medical bacteria, 3rd edn, Williams and Wilkins, Philadelphia, P. A; 2000.
Madigan MT, Martinko JM. Brock Biology of Microorganisms, (12th ed.) Benjamin Cummings, Upper Saddle River, NJ; 2008.
Mahon CR, Lehman DC and Manuselis G. Textbook of diagnostic microbiology. 4th (ed.) Saunders, W. B. Co., Philadelphia, PA; 2011.
Yamada R, Tanaka T, Ohnishi Y, Suematsu K, Minami M, Seki T, et al. Identification of 142 single nucleotide polymorphisms in 41 candidate genes for rheumatoid arthritis in the Japanese population. Human Genetics. 2000;106: 293–297.
Katsura K, Kawasaki H, Potacharoen W, Saono S, Seki T, Yamada Y, et al. Asaia siamensis sp. nov., an acetic acid bacterium in the α-proteobacteria. International Journal of Systematic and Evolutionary Microbiology. 2001;51:559–563.
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–2729.
Odokuma LO, Dickson AA. Bioremediation of a crude oil-polluted tropical mangrove environment. Journal of Applied Science and Environmental Management. 2003;7: 23-29.
Chikere CB, Ekwuabu CB. Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria. African Journal of Environmental Science and Technology. 2014;8(6):401-406
Omoregbee O, Ikhajiagbe B, Udinyiwe C. O. Studies of heavy metal contents and microbial composition of the rhizosphere of Panicum maximum within and around auto mechanic workshop in Benin City. Journal of Applied Science Environmental Management. 2015;19(2):271–276.
Borowik A, Wyszkowska J, Gałązka A, Kucharski J. Role of Festuca rubra and Festuca arundinacea in determining the functional and genetic diversity of microorganisms and of the enzymatic activity in the soil polluted with diesel oil. Environmental Science and Pollution Research. 2019;26(27):27738-27751.
Efeovbokhan VE, Hymore FK, Oyakhire, CTG. The Effects of Pseudomonas aeroginosa and Aspergillus niger on the bioremediation of raw and treated crude oil-polluted water. International Journal of Science and Technology. 2012;2(6):2224-3577.
Romero MC, Hammer E, Cazau MC, Arambarri AM. Selection of autochthonous yeast strains able to degrade biphenyl. World Journal of Microbiology and Biotechnology. 2001;17(6):591-594.
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